Other

Part:BBa_K1093023:Design

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-23)


MinC/Lysis device-based kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3067
    Illegal NheI site found at 3090
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1224
    Illegal AgeI site found at 2672
    Illegal AgeI site found at 2742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3323


Design Notes

Part is nearly identical to BBa_K1120808 regulated by BBa_K098995. The only difference is MinC coding sequence inserted before t gene


Source

The sequence was synthetized by GeneRay

References